Aspectos da relação interpessoal entre estagiários e professores da educação básica: uma análise dos discursos atitudinais de afeto em relatos de alunos- mestres.

This research discusses the interpersonal relationship between trainees and Basic Education teachers, using as theoretical support the EVALUATION System from Halliday's Functional Systemic Linguistics (FSL), with the focus on the attitudinal discourses of affect in internship reports of the Language Arts Course. The way relationships develop in the school environment is of fundamental importance in the interaction between student-teacher and teacher, exerting influence on this dichotomy of practice-theory and theory-practice. The general objective is to analyze the aspects of the interpersonal relationship between trainees and Basic Education teachers, through the EVALUATION system. As for the specific objectives, we analyze the lexicon anchored in the evaluation system; discuss the relationship between the trainees and Basic Education teachers and identify in the textual productions of the trainees the discourse of affection. The research approach is qualitative, being supported by authors who address the theme of this research. The research is also documentary, as it uses a corpus consisting of 10 reports from Supervised Internship produced by undergraduate students of the Language Arts Course of the Federal University of Tocantins (UFT) Araguaína campus. These reports were processed in the IRAMUTEQ software and were also performed thorough scan by the researcher to locate discursive clippings that we call fragments. After analyzing the fragments grounded by Martin and White (2005) and Martin and Rose (2013) EVALUATION System, we found out that the way trainees were received by the Basic Education teachers in this phase of the Internship produced feelings of insecurity and unhappiness, which lead us to say that this first phase of Supervised Internship is sometimes uncomfortable for the trainees.Essa pesquisa discorre a respeito da relação interpessoal entre estagiários e professores da educação básica, utilizando como suporte teórico o Sistema de AVALIATIVIDADE da Linguística Sistêmica Funcional (LSF) de Halliday (1967) com o foco nos discursos atitudinais de Afeto em relatórios de estágio do Curso de Letras. A forma como as relações se desenvolvem no ambiente escolar são de fundamental importância na interação entre aluno-mestre (AM) e professor, exercendo influência nesta dicotomia da teoria-prática e prática-teoria. O objetivo geral é analisar os aspectos da relação interpessoal entre estagiários e professores da educação básica, através do sistema de AVALIATIVIDADE da LSF. Quanto aos objetivos específicos, eles são analisar o léxico ancorado no sistema de avaliatividade; discorrer a respeito de como acontece a relação entre o aluno-mestre e o professor da Educação básica (EB) e identificar nas produções textuais dos alunos-mestres (AM) o discurso do afeto. A abordagem de pesquisa utilizada é a qualitativa, sendo fundamentada por autores que abordam o tema desta pesquisa. Também a pesquisa é documental, pois utiliza o corpus constituído por 10 relatórios de Estágio Supervisionado Curricular (ESC) produzidos por graduandos do Curso de Letras da Universidade Federal do Tocantins (UFT), câmpus de Araguaína -TO. Os RES foram processados no software IRAMUTEQ e também foi realizada uma varredura minuciosa pelo pesquisador para localizar recortes discursivos que chamamos de fragmentos. Depois de analisados os fragmentos fundamentados pelo Sistema de AVALIATIVIDADE de Martin e White (2005) e Martin e Rose (2013) constatamos que a forma como o AM, foi recebido pelo professor da EB nesta fase do RES, produziu sentimentos de insegurança e infelicidade, o que nos leva a dizer, baseado nos dados pesquisados, que esta primeira fase do ESC é por vezes desconfortável para o AM

Aspectos da relação interpessoal entre estagiários e professores da educação básica: uma análise dos discursos atitudinais de afeto em relatos de alunos- mestres.

This research discusses the interpersonal relationship between trainees and Basic Education teachers, using as theoretical support the EVALUATION System from Halliday's Functional Systemic Linguistics (FSL), with the focus on the attitudinal discourses of affect in internship reports of the Language Arts Course. The way relationships develop in the school environment is of fundamental importance in the interaction between student-teacher and teacher, exerting influence on this dichotomy of practice-theory and theory-practice. The general objective is to analyze the aspects of the interpersonal relationship between trainees and Basic Education teachers, through the EVALUATION system. As for the specific objectives, we analyze the lexicon anchored in the evaluation system; discuss the relationship between the trainees and Basic Education teachers and identify in the textual productions of the trainees the discourse of affection. The research approach is qualitative, being supported by authors who address the theme of this research. The research is also documentary, as it uses a corpus consisting of 10 reports from Supervised Internship produced by undergraduate students of the Language Arts Course of the Federal University of Tocantins (UFT) Araguaína campus. These reports were processed in the IRAMUTEQ software and were also performed thorough scan by the researcher to locate discursive clippings that we call fragments. After analyzing the fragments grounded by Martin and White (2005) and Martin and Rose (2013) EVALUATION System, we found out that the way trainees were received by the Basic Education teachers in this phase of the Internship produced feelings of insecurity and unhappiness, which lead us to say that this first phase of Supervised Internship is sometimes uncomfortable for the trainees.Essa pesquisa discorre a respeito da relação interpessoal entre estagiários e professores da educação básica, utilizando como suporte teórico o Sistema de AVALIATIVIDADE da Linguística Sistêmica Funcional (LSF) de Halliday (1967) com o foco nos discursos atitudinais de Afeto em relatórios de estágio do Curso de Letras. A forma como as relações se desenvolvem no ambiente escolar são de fundamental importância na interação entre aluno-mestre (AM) e professor, exercendo influência nesta dicotomia da teoria-prática e prática-teoria. O objetivo geral é analisar os aspectos da relação interpessoal entre estagiários e professores da educação básica, através do sistema de AVALIATIVIDADE da LSF. Quanto aos objetivos específicos, eles são analisar o léxico ancorado no sistema de avaliatividade; discorrer a respeito de como acontece a relação entre o aluno-mestre e o professor da Educação básica (EB) e identificar nas produções textuais dos alunos-mestres (AM) o discurso do afeto. A abordagem de pesquisa utilizada é a qualitativa, sendo fundamentada por autores que abordam o tema desta pesquisa. Também a pesquisa é documental, pois utiliza o corpus constituído por 10 relatórios de Estágio Supervisionado Curricular (ESC) produzidos por graduandos do Curso de Letras da Universidade Federal do Tocantins (UFT), câmpus de Araguaína -TO. Os RES foram processados no software IRAMUTEQ e também foi realizada uma varredura minuciosa pelo pesquisador para localizar recortes discursivos que chamamos de fragmentos. Depois de analisados os fragmentos fundamentados pelo Sistema de AVALIATIVIDADE de Martin e White (2005) e Martin e Rose (2013) constatamos que a forma como o AM, foi recebido pelo professor da EB nesta fase do RES, produziu sentimentos de insegurança e infelicidade, o que nos leva a dizer, baseado nos dados pesquisados, que esta primeira fase do ESC é por vezes desconfortável para o AM

A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis

Background: Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium Corynebacterium pseudotuberculosis, the etiological agent of caseous lymphadenitis (CLA) in sheep and goats. Results: An optimized protocol of three-phase partitioning (TPP) was used to obtain the C. pseudotuberculosis exoproteins, and a newly introduced method of data-independent MS acquisition (LC-MSE) was employed for protein identification and label-free quantification. Additionally, the recently developed tool SurfG+ was used for in silico prediction of sub-cellular localization of the identified proteins. In total, 93 different extracellular proteins of C. pseudotuberculosis were identified with high confidence by this strategy; 44 proteins were commonly identified in two different strains, isolated from distinct hosts, then composing a core C. pseudotuberculosis exoproteome. Analysis with the SurfG+ tool showed that more than 75% (70/93) of the identified proteins could be predicted as containing signals for active exportation. Moreover, evidence could be found for probable non-classical export of most of the remaining proteins. Conclusions: Comparative analyses of the exoproteomes of two C. pseudotuberculosis strains, in addition to comparison with other experimentally determined corynebacterial exoproteomes, were helpful to gain novel insights into the contribution of the exported proteins in the virulence of this bacterium. The results presented here compose the most comprehensive coverage of the exoproteome of a corynebacterial species so far

Serological secretome analysis of Corynebacterium pseudotuberculosis

Caseous lymphadenitis (CLA) is a chronic disease affecting small ruminants caused by Corynebacterium pseudotuberculosis and is responsible for significant economic losses. Previous studies have reported that a set of C. pseudotuberculosis secreted proteins react with sera from infected goats. Mapping of the secretome will shed light on the pathogenesis of CLA. We have hereby identified six immunoreactive secreted proteins of C. pseudotuberculosis by 2D Western blotting using sera from goats with CLA and characterized them by mass spectrometry. The information retrieved will support studies leading to the development of efficient vaccines and diagnostic kits